The Alliance Technologies and Resources for Research Core Laboratories (TRRCL) bridges the gap in essential technical resources by: supporting research training for investigators and trainees, providing continued training and education for core directors and staff; and providing technical expertise and services for investigators across the Alliance.

The Core ensures a greater integration of training and professional development activities with the Alliance’s Professional Development Core (PDC) across the partner institutions through a novel Training Organization Committee. TRRCL is innovative because it extends core services to include the IDeA National Resource for Proteomics in Oklahoma and Arizona INBREs and will integrate the existing TRRCL research cores with other INBRE and COBRE Cores in Puerto Rico.

Our goal is to improve joint research facilities for investigators pursuing clinical and translational research, to improve health in under-resourced PR communities.

  • Specific Aim 1. Support research training for faculty investigators and other trainees pursuing clinical and translational research and provide continued training and education for core directors and technical support staff.
  • Specific Aim 2. Provide technical expertise and services for investigators conducting clinical and translational research.
  • Specific Aim 3. Increase TRRCL´s visibility and impact through access to diverse core services and community participation for clinical and translational investigators.
  • Research Training Seminars Program: genomic translational research and cancer-related
  • Training Workshops
  • Research Training Opportunity Program (RTOP).
  • Early Stage Career Investigator Development Program (ESCID)
  • Promote use of TRRCL resources by clinical and translational researchers in Puerto Rico
  • Cost Recovery System
  • Community Outreach Activities
  • Collaboration with Biospecimens Repositories

The TRRCL team will conduct regular needs assessment surveys to determine what technology, training, and/or instrumentation is needed by Alliance investigators to conduct their research.

Our Services

The Technologies and Resources for Research Core Laboratories (TRRCL) will address the IDeA-CTR core areas by providing research support facilities, training, access to research infrastructure in the Alliance institutions, and by facilitating access to research cores and technical support at other IDeA locations in the U.S. mainland. By using these NIH resources to leverage and strengthen CTR interactions in Puerto Rico, TRRCL will continue to contribute to increase diversity among the institutions and researchers conducting CTR in the US.

To see the list of TRRCL Active Projects click here

SERVICES

  • Centrifuge
  • Prepare slides
  • Storage (room temperature, refrigerated, frozen)
  • Packing
  • Shipping (room temperature, dry ice)
  • Preservation of samples (with diluents, buffers, etc.)
  • Process serum samples for Comprehensive Metabolic Panel
  • Process serum samples for Basic Metabolic Panel
  • Process serum samples for Lipid Panel
  • Process whole blood samples for HbA1c
  • Process whole blood samples for CBC (Complete Blood Count) and 5-part Differential
  • Process urine samples for Urinalysis
  • Process urine samples for Microalbumin
  • Process urine samples for Pregnancy Test

EQUIPMENT AVAILABLE:

  • Vitros 250 (Routine Chemistry)
  • Hood
  • Microscope
  • Incubators
  • Refrigerators
  • Freezers
  • Water Baths
  • Clinitek 500 (Urinalysis)
  • DCA 2000+ (HbA1c and Microalbumin)
  • Refrigerated Centrifuges
  • Micro-centrifuge
  • AcT 5diff CP (Hematology)

Lab Technician

The Genomic Translational Research Unit (GTRU) is located within the Puerto Rico OMIC Center (PROMIC) at the UPR Comprehensive Cancer Center. GTRU serves clinical and translational researchers from the medical genetics faculty affiliated with the Newborn Screening Program at the UPR Pediatric Hospital, clinical faculty conducting research in cancer diagnostics and cancer treatment at the UPR Comprehensive Cancer Center (UPRCCC), and UPR-MSC biomedical faculty conducting human genetics and pharmacogenomics research.

The GTRU resources have been combined with those of the RCMI Center for Genomics in Health Disparities and Rare Diseases and the PR-INBRE Core Resources and Facilities to provide more comprehensive resources and technologies.

SERVICES

  • DNA extraction and QC
  • RNA extraction, cDNA template preparation, and QC
  • Library Preparation for Sequencing, Templates preparation
  • DNA Sequencing Reactions (type of service: whole genome, whole exome, transcriptome (RNAseq), ChIPSeq, other)
  • Processing of raw data for subsequent bioinformatics analyses by investigator

The Molecular Biology Core Laboratory at PMSF has include a research service for the acquisition of preliminary data by early stage investigators from all Alliance institutions, many of whom do not have assigned research space, start-up funds, or technical support.

SERVICES

  • Mammalian tissue culture,
  • Cell transfection/ transformation
  • DNA, RNA, and protein isolation
  • Cell cryostorage
  • Expert consultation in molecular biology experimental design. These services do not and will not overlap with RCM
  • Development of animal models for human diseases
  • Histology tissue in paraffin embedding

EQUIPMENT AVAILABLE

  • Gel and Blot Imaging Systems
  • Typhoon 7000 FLA, GE
  • Epi Chemi II Darkroom, UVP
  • Thermal Cycler and Q-PCR Systems
  • DNA Engine, BIO-RAD
  • DNA Engine DYAD, MJ Research
  • iCycler, BIO-RAD
  • Realplex2, Eppendorf
  • Eco, Illumina
  • Nucleic Acid and Protein
  • Quantification and Analysis
  • Biophotometer, Eppendorf
  • NanoDrop 2000, Thermo Sci.
  • Experion, BIO-RAD
  • Microscope and Cell Analysis
  • Cellometer K2, Nexcelom
  • Evos Fl, Life Technologies
  • BX60 microscope, Olympus
  • Multimode Plate Reader
  • Synergy HT, BioTek
  • Tissue and Sample Dissociators
  • gentleMACS Disruptor, Miltenyi
  • Bullet Blender Storm, Next Advance
  • Sonicator, Fisher
  • Histo Core

The Histopathology Core Laboratory at UCC has been developed with Alliance funding, leveraging equipment and expertise already at UCC, providing standard and customized research-specific histology.

SERVICES

  • Paraffin embedding, sectioning, and preparation of frozen sections
  • Routine and special histological stains
  • Immunohistochemistry, and immunofluorescence
  • Coordinating the services of a consulting pathologist who analyze and interpret tissue sections of histopathology-related studies when necessary

EQUIPMENT AVAILABLE

  • FACSCanto II
  • Class II Safety Cabinets
  • Refrigerators
  • Thermocycles
  • Repository for Lymphocytes and Plasma
  • Centrifuges
  • CO2-incubators
  • Ultrafreezer
  • iCycler iQ Real-Time PCR
  • Elispot

Standard Operation Procedures

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jporter@psm.edu

Phone: (787) 840-2575 Ext. 2251

James Porter, PhD

Leader
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denisse.melecio@upr.edu

Phone: 787-759-0306 Ext.242

Denisse Melecio, MBA

Coordinator
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cchardon@psm.edu

Phone (787) 840-2575 Ext. 2200

Camille Chardón, MS

Research Laboratory Technician
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Phone: (787)758-2525 Ext. 2299

José Rodríguez Medina, PhD

MSC Co-Leader
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nilda.gonzalez1@upr.edu

787-759-0306 Ext. 226

Nilda González, MS, MT

Lab Manager
Misty Eaton

misty.eaton@uccaribe.edu

Phone:(787) 798-3001 Ext. 2034

Misty Eaton, PhD

UCC Co-Leader
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carola.lopez-cepero@upr.edu

787-759-0306 Ext. 226

Carola López-Cepero, MS, MT

Research Technologist